seabet time:April 20247Day (week日）morningStart at 10 o'clock
Reporting location: Computer Building of the school headquarters313
seabet title: Analysis of dynamic effects of anti-tumor drugs at the single cell level
seabet summary: Gemcitabine is commonly used in pancreatic ductal adenocarcinoma (PDAC），However, chemotherapy resistance is still a problem in the treatment of PDAC。Understanding cellular heterogeneity and dynamic plasticity is critical to overcoming tumor drug resistance。Single seabet technology enables analysis of seabet status under specific conditions or time points，But how to connect the "snapshots" of tumor cells at each time point into a dynamic picture is still very challenging。The research team used scRNA-seq technology to detect the PDAC heterologous transplant model，To study the complex cellular responses associated with gemcitabine resistance。To reconstruct the dynamic response of tumor cells from perturbed scRNA-seq data，We proposed scConGraph scalable two-layer graph model，Can effectively integrate cross-temporal contextual similarity information to calculate vector representations of cells，Then define seabet subpopulations in the representation space、Align seabet state fate。Based onscConGraph，We observed that stem seabet characteristics and endoplasmic reticulum stress contribute significantly to primary drug resistance。Meanwhile，Before and after gemcitabine treatment，Different patterns of changes according to genetic differences，seabet subpopulations can be divided into seabet cycle type I、seabet cycle type II、Silent and epithelial-mesenchymal transition (EMT) types。Further analysis found that gemcitabine causes upregulation of GDF15，Especially in the resting subpopulation, the upregulation is more obvious。Experimental confirmation，Inhibiting GDF15 increases the sensitivity of tumor cells to gemcitabine，This suggests that GDF15 is a potential target for gemcitabine-induced chemotherapy resistance。
About the speaker: Gu Jin，Department of Automation, Tsinghua University，Permanently appointed associate professor。The seabet team aims at key problems in analyzing the genetic code of life and the regulation laws of complex systems，Develop new information theory and intelligent technology，Promote precision and individualization of diagnosis and treatment of major diseases such as malignant tumors，Already hereNature seabet Biology、seabet Stem seabet、GPB、Published more than 50 papers in Bioinformatics and other magazines。Currently Secretary General of the Intelligent Health and Bioinformatics Committee of the Chinese Society of Automation、Standing Member of the Bioinformatics and Artificial Intelligence Professional Committee of the Chinese Artificial Intelligence Society。Host the National Natural Science Foundation of China Outstanding Youth Science Fund、Key projects、National key research and development plan projects and other national scientific research projects。
